Association between vitamin D receptor BsmI (rs1544410) gene polymorphism and serum vitamin D, serum calcium, and osteoporosis risk in women over 40 years

Articles in Press

Document Type : Original Article

Authors

1 Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran

2 Department of Orthopedics, Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran

3 Assistant Professor of Rheumatology, Department of Internal medicine, Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran

Abstract

Objectives: We investigated the relationships between the vitamin D receptor BsmI (rs1544410) gene polymorphism, serum vitamin D and calcium levels, and the risk of osteoporosis in women aged 40 years and older.
 
Methods: This case-control investigation enrolled participants (30 cases and 30 controls) classified into normal and osteoporotic groups using a dual-energy X-ray absorptiometry (DEXA) scan. Vitamin D and calcium levels were obtained from peripheral blood samples. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to detect the BsmI polymorphism. Differences between groups were analyzed using an analysis of variance (ANOVA) test.
 
Results: 60 individuals (30 cases and 30 age-matched controls) were enrolled. The lumbar and femoral T-scores, as well as calcium levels, were significantly lower in the cases compared to the controls (P < 0.005). Among postmenopausal women, the lumbar T-scores significantly differed between cases with VDR polymorphisms (P = 0.003) and controls (P = 0.001). Among premenopausal women, there was only a significant difference in VDR polymorphisms in the control group (P = 0.006). Calcium and vitamin D levels were significantly different between the VDR polymorphisms in cases and control groups, but only among postmenopausal women (P < 0.005 for all comparisons). No significant differences were observed between the VDR polymorphism in terms of femoral T-scores, neither in the cases nor the controls (P for all > 0.005).
 
Conclusion: This study found no differences in VDR polymorphism between individuals with and without osteoporosis. Additionally, the lumbar T-scores, calcium, and vitamin D levels were significantly different between the VDR polymorphisms in the case and control groups among postmenopausal women.
 
 
 

Keywords

Acknowledgments: The data and result is extracted from a thesis. We would like to thank the participants and the staff of Islamic Azad University for their support of this project.

 

Availability of data and materials: The datasets used in this study are available from the corresponding author upon reasonable request.

 

Conflicts of interests: All authors declared that they have no competing interests.

 

Consent for publication: Not applicable.

 

Ethics approval and consent to participate: The study protocols were approved by the Ethics Committee of Islamic Azad University, Mashhad, Iran, with the ethics code IR.IAU.MSHD.REC.1399.053.The study was conducted in accordance with the ethical principles outlined in the Declaration of Helsinki.

 

Financial disclosure: No financial support was received for this study.

 

Author contributions: Each author has made substantial contributions to the final manuscript, approved the submitted version, and agreed to be personally accountable for the author's contributions and to ensure that questions related to the accuracy or integrity of any part of the work.

 

Open Access Policy: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/

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Razavi International Journal of Medicine

Articles in Press, Accepted Manuscript
Available Online from 08 July 2025

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