Evaluation of Efficacy and Potential Complications of the Plant-Based Nanoparticles Hemostat Powder on Controlling Hemorrhage in Rat Models of Liver Injury: An Experimental Study

Document Type : Original Article


1 Associate professor of cardiac surgery, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

2 General Physician, Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran

3 Assistant professor, Department of cardiac surgery, Faculty of medical science. Mashhad University of Medical Sciences, Iran.

4 Researcher in education and research department of Razavi Hospital .Mashhad. Iran

5 Resident of cardiac surgery, Department of cardiac surgery, Faculty of medical science, Mashhad University of Medical Sciences .Iran



Liver surgery is associated with an increased risk of bleeding, which required time management. Several hemostatic agents have been developed. However, plant-based materials have shown some superiority in hemostasis time and lower complications. The aim of our study was to prepare plant-based topical hemostatic Nanoparticles from the rosaceous family as a novel hemostatic agent.
Material and Methods
Twenty male Wistar rats were randomly divided into two study groups. All the rats were sedated and underwent incision in the anterior lobe of the liver. In the control group, the incision site was firstly compressed with a manual compression with dry gauze for 2 minutes and then repeated every 30 seconds until hemostasis was achieved. In the intervention group, homeostatic material was applied on the site of the incision, and clotting time was recorded. Paired Samples Test was used to compare the mean hemo­stasis times in Nanoparticles and control groups. P <0.05 considered significant.
Hemostasis was successfully achieved in both groups. Hemostasis time in Nanoparticles and control groups were 6.7±1.33 and 183±26.26 seconds, respectively and their differences were significant (P < 0.001). The histopathology assessment showed mild increases in vascularity, fibrosis, and polymorph nuclear neutrophils (PMN) infiltration was seen in one case. Mild to moderate lymphocyte infiltration was detected in 2 cases.
The produced plant-based Nanoparticles could significantly reduce the hemostasis time compared to the control group.


 1.Mallett S, Sugavanam A, Krzanicki D, Patel S,
Broomhead R, Davidson B, et al. Alterations in
coagulation following major liver resection.
2.Xi C, Zhu L, Zhuang Y, Wang S, Sun G, Liu Y, et al.
Experimental evaluation of tranexamic acid–loaded
porous starch as a hemostatic powder. 2018;24(2):279-
3.Bennett BLJW, medicine e. Bleeding control using
hemostatic dressings: lessons learned. 2017;28(2):S39-
4.Khoshmohabat H, Paydar S, Kazemi HM, Dalfardi
BJTm. Overview of agents used for emergency
hemostasis. 2016;21(1).
5.Emilia M, Luca S, Francesca B, Luca B, Paolo S,
Giuseppe F, et al. Topical hemostatic agents in surgical
practice. 2011;45(3):305-11.
6.Schwartz RB, Reynolds BZ, Shiver SA, Lerner EB,
Greenfield EM, Solis RA, et al. Comparison of two
packable hemostatic Gauze dressings in a porcine
hemorrhage model. 2011;15(4):477-82.
7.Kelly JF, Ritenour AE, McLaughlin DF, Bagg KA,
Apodaca AN, Mallak CT, et al. Injury severity and
causes of death from Operation Iraqi Freedom and
Operation Enduring Freedom: 2003–2004 versus 2006.
8.Hangge P, Stone J, Albadawi H, Zhang YS,
Khademhosseini A, Oklu RJCd, et al. Hemostasis and
nanotechnology. 2017;7(Suppl 3):S267.
9.Sher Z, Khan Z, Hussain F. Ethnobotanical studies of
some plants of Chagharzai valley, district Buner,
Pakistan. Pak J Bot. 2011;43(3):1445-52.
10.Pala NA, Negi A, Todaria N. Traditional uses of
medicinal plants of Pauri Garhwal, Uttrakhand. Nature
and Science. 2010;8(6):57-61.
11.Hazrat A, Nisar M, Shah J, Ahmad S. Ethnobotanical
study of some elite plants belonging to Dir, Kohistan
valley, Khyber Pukhtunkhwa, Pakistan. Pak J Bot.
12.Mari A, Lyon D, Fragner L, Montoro P, Piacente S,
Wienkoop S, et al. Phytochemical composition of
Potentilla anserina L. analyzed by an integrative GC-MS
and LC-MS metabolomics platform. Metabolomics.
13.Xue P-F, Luo G, Zeng W-z, Zhao Y-Y, Liang H.
Secondary metabolites from Potentilla multifida
L.(Rosaceae). Biochemical systematics and ecology.
14.Satar NYG, Akkoc A, Oktay A, Topal A, Inan K.
Evaluation of the hemostatic and histopathological
effects of Ankaferd Blood Stopper in experimental liver
injury in rats. Blood Coagulation & Fibrinolysis.
15.Ozemir IA, Bilgic C, Aytac E, Aslan S, Bayraktar B,
Isbilen B, et al. Application of solid Carbon dioxide as a
novel hemostatic agent on a hepatectomy model in rats.
Biomedical Research. 2016;27(3):860-6.
16.Midi A, Ozyurek HE, Karahan S, Ekici H, Kumandas
A, Turkmen I, et al. Investigation of efficacy of the plant
based algan hemostatic agent in hepatectomy bleeding
model in rats. EJMI. 2018;2:195-201.
17.Abajy MY, Nayal R, Takla S. Investigation of the
Effect of Potentilla erecta and Plantago lanceolata on
Bleeding Time in Mice.