Assessment the of Amplification HER-2/neu Gene by Chromogenic In Situ Hybridization (CISH) Compared to Immunohistochemistry (IHC) Method in Gastric Cancer


1 Student Research Committee, Gastrointestinal and Fatty Liver Research Center, Faculty of Medical Sciences,Mashhad University of Medical Sciences,Mashhad, IR Iran

2 Fellowship of Molecular Pathology and Cytogenetics, ImamReza Hospital,Mashhad University of Medical Sciences,Mashhad, IR Iran

3 Fellow of Molecular Pathology and Cytogenetics, Shiraz University of Medical Sciences, Shiraz, Iran

4 Professor of Pathology, ImamReza Hospital,Mashhad University of Medical Sciences,Mashhad, IR Iran

5 Resident of pathology, ImamReza Hospital,Mashhad University of Medical Sciences,Mashhad, IR Iran


Abstract Background: Gastric cancer has a highmortality rate and often has a poor treatment outcome. The HER2/neu gene target therapy has been known as a potential way for treatment. Objectives: The goal of our study was assessment the relation between chromogenic in situ hybridization (CISH) and immunohis- tochemistry (IHC)methods in determining the best diagnosticmethod for gastric cancer. Methods: In this historical cohort study, 50 gastric cancer samples were analyzed by CISH and IHC. The relation between clinical- pathological parameters of HER2/neu was also analyzed. Alive patients were followed from 2009 through 2012 for the main out- comes (mortality). The results of these twomethods, in terms of sex, age, tumor size, grading, staging, tumor location,metaplasia, presence of necrosis and ulceration, vascular invasion, the TNM system, mucin or signet producing adenocarcinoma cells and pa- tient survival rates were compared. Results: There was no significant difference between IHC and CISH regarding the sex, age, tumor size, grading, staging, tumor lo- cation,metaplasia, presence of necrosis and ulceration, vascular invasion, the TNMsystem,mucin or signet producing adenocarci- noma cells and patient survival rates. Comparison of TNMscores by these twomethods showed no significant relationship between IHC and staging, but a statistically significant difference between CISH and different N staging, (P < 0.05) was assessed. Conclusions: Comparison between IHC and CISH showed the only significant relationship between CISH and different N staging. Therefore, low amplified CISH was a better diagnosticmethod for gastric cancer, compared to low expression in IHC.


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