Evaluation of the Cytotoxic Effect of Tissue Glue (Octyl 2-Cyanoacrylate) on H9C2 Cardiomyoblast Cells Using Extract Dilution Assay


1 Department Pediatrics Cardiology, Mashhad Branch, Islamic Azad University, Mashhad, IR Iran

2 Department of Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

3 Department of Biostatistics, Mashhad Branch, Islamic Azad University, Mashhad, IR Iran

4 Innovative Medical Research Center, Department of Biochemistry, Mashhad Branch, Islamic Azad University, Mashhad, IR Iran



Background: Recently, Tissue Glue or Octyl 2-Cyanoacrylate (OCA) has been extensively used in cardiac surgeries as surgical adhesives to replace sutures. Since the binder is in contact with live tissues, its tissue compatibility and non-cytotoxicity have major effect on enhancing the healing process. Objectives: The purpose of this study was to evaluate the cytotoxicity of OCA on rat cardiomyoblast cell (H9C2) using MTT assay. Materials and Methods: Cells were cultured in the presence of various concentrations of the OCA extract. Then, morphological changes of the cultured cells were observed by light microscopy and their cytotoxicity by MTT assay. The morphology quality of the cells and their quantity were examined after 24- hour incubation up to 72 hours. Data were analyzed statistically by the ANOVA. Results: The results showed that the percentage of survived cells amount of extract dilution ratio (1:4; 1:2; 1) were 80.4%; 79.6%, 77.5% respectively. Analysis of statistical test results does not show any significant differences between the optical density of the extract dilutions, and the control group indicating; consequently it has no significant cytotoxicity effect on the cells. Conclusions: The outcome of this study is that the OCA has no toxic effect on H9C2 cells.