Erythropoietin Effects on Pathological Changes of Brain Tissues and Motor Balance Functions after Traumatic Brain Injury in Animal Model


1 Department of Neurosurgery, Emdad Academic Hospital, School of Medicine,Mashhad University of Medical Sciences,Mashhad, IR Iran

2 Ph.D. Candidate of Medical Biochemistry, Department of Biochemistry, Faculty of Medicine,Mashhad University of Medical Sciences,Mashhad, IR Iran



Abstract Objectives: In this control trial study we showed the neuroprotective effects of the erythropoietin (EPO) in traumatic brain injury in animalmodel. Methods: The researchwas carried out on 50maleWistar ratsweighing 200 to 250 g. Theywere divided into two groups of control andcase. The ratswere anesthetized, right frontal craniotomywasperformedandthenthebraindamagewas causedbyweight-drop model. In the case group, after 3 hours and again after 24 hours of craniotomy and creation of brain lesions in the right hemisphere, 500 u/kg erythropoietinwas injected into peritoneumbut in the control group, the rats did not receive any drug. Then the behavior, motor function and balance on the second day and the fourteenth day after injection of erythropoietin were evaluated. After that, the rats were killed and the brain tissues sent to the laboratory for pathological assessment of brain tissues. Results: The average of cross-sectional damage in the case group that received erythropoietin drug was reported 22.55% and in the control group 37.41%, and themotor balance function after fourteenth day in the group that received erythropoietinwas 69.12% and better in comparison with the control group (46.27%) that did not receive any drug. Conclusions: erythropoietin has a protective effect on neurons and improves the sub-acute changes in head after brain injury and increases themotor balance abilities in rats.